服务平台拥有丰富的技术服务经验，一流的仪器设备，先进的检测手段和严格的质量控制体系，力求为全球药物研发机构提供优质高效的技术服务
背景介绍 Matrix metalloproteinase 3 (MMP3) is a zinc-dependent endopeptidase involved in the breakdown of extracellular matrix and tissue remodeling in normal physiological processes, such as embryonic development and reproduction, as well as in disease processes, such as arthritis and cancer. Overexpression of MMPs is associated with tumor invasion, neoangiogenesis and metastasis. Several inhibitors of MMPs (MMPI), small molecules and blocking monoclonal antibodies, have entered clinical trials. MMP3 (K45E) variant is associated with differences in MMP3 activity and has been linked to cancer susceptibility. 产品介绍 The MMP3 (K45E) Inhibitor Screening Assay Kit is designed to measure the endopeptidase activity of MMP3 (K45E) for screening and profiling applications.
询价产品介绍 ATP citrate lyase (ACLY) is an important enzyme in fatty acid synthesis and cancer metabolism. The ACLY Assay Kit is designed to measure ACLY activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.
询价产品介绍 UBCH5c (UBE2D3) is an E2 conjugating ubiquitin protein that functions in the ubiquitination of the tumor-suppressor protein p53. It is a promising drug target in cancer immunotherapy. The UBCH5c TR-FRET Assay Kit is designed to measure ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled Ubiquitin and a terbium-labeled antibody recognizing the His-tagged UBCH5c protein to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.
询价产品介绍 UBCH5a (UBE2D1) is an E2 conjugating ubiquitin protein that functions in the ubiquitination of the tumor-suppressor protein p53. It is a promising drug target in cancer immunotherapy. The UBCH5a TR-FRET Assay Kit is designed to measure ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled Ubiquitin and a terbium-labeled antibody recognizing the His-tagged UBCH5a protein to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.
询价产品介绍
The Cereblon Binding Assay Kit (FP) is designed for testing and profiling of Cereblon (CRBN) inhibitors using Fluorescence Polarization. The assay is a competitive binding assay, based on the binding of fluorescently-labeled Thalidomide, a CRBN binder, to purified recombinant CRBN.
CRBN is the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. CRBN is a direct protein target for the immunomodulatory and antiproliferative activities of thalidomide analogues such as lenalidomide and pomalidomide.
The Cereblon Binding Assay Kit (FP) comes in a convenient 96-well format, with enough purified CRBN, Cy5-labeled Thalidomide, and assay buffer for 100 enzyme reactions. In addition, the kit includes the CRBN inhibitor Pomalidomide for use as an inhibitor control.
The key to the CRBN Assay Kit is the fluorescently-labeled Thalidomide. Using this kit, only one simple step on a microtiter plate is required for CRBN reactions. The Cy5-labeled Thalidomide is incubated with a sample containing CRBN to produce a change in fluorescent polarization that can be measured using a fluorescence reader.
背景介绍 Mouse RIPK1 is a member of the receptor-interacting protein kinase (RIP) family. It plays an important role in cell survival as well as cell death, including apoptosis and necroptosis. RIPK1 is highly expressed in human Alzheimer's disease (AD) brains and in AD mouse models. Importantly, it was shown that inhibition of RIPK1 results in decreased amyloid and inflammation levels as well as accelerated amyloid beta peptide (Aβ) degradation by microglia in vitro. 产品介绍 The Mouse RIPK1 Kinase Assay Kit is designed to measure Mouse RIPK1 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.
询价产品介绍 UBCH5b (UBE2D2) is an E2 conjugating ubiquitin protein that functions in the ubiquitination of the tumor-suppressor protein p53. It is a promising drug target in cancer immunotherapy. It utilizes biotin-labeled Ubiquitin and a terbium-labeled antibody recognizing the His-tagged UBCH5b protein to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.
询价背景介绍 IL-17A is a pro-inflammatory cytokine produced by TH17 cells that plays a key role in inflammation, autoimmunity, and host defense. It has been shown to stimulate the production of pro-inflammatory cytokines, namely IL-6 and IL-8, by synoviocytes. Due to its role as a mediator of inflammation, the IL-17 pathway has become a popular target for treating diseases that have a strong inflammatory component such as psoriasis, arthritis, Crohn's disease, and ankylosing spondylitis. 产品介绍 The IL-17RA:IL-17A[Biotin] Inhibitor Screening Assay Kit is designed for screening inhibitors of IL-17A:IL-17RA interaction. The key to this kit is the high sensitivity of detection of biotin-labeled IL-17A by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, IL-17RA is coated on a 96-well plate. Next, IL-17A[Biotin] is incubated with IL-17RA on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
询价背景介绍 c-KIT is a proto-oncogene and a type III transmembrane receptor for mast cell growth factor, also known as stem cell factor. It plays an essential role in the regulation of cell survival and proliferation, as well as hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and mast cell development, migration and function. Activating mutations in cKIT are associated with gastrointestinal stromal tumors (GIST), testicular seminoma, mast cell disease, melanoma, and acute myeloid leukemia, while inactivating mutations are associated with the genetic defect piebaldism. Secondary point mutation V654A, a commonly acquired mutation within the cKIT ATP-binding pocket of the kinase domain, has been found in patients with GIST undergoing treatment with Imatinib. The V654A mutation is strongly correlated with Imatinib resistance and rapid progression of GISTs. 产品介绍 The cKIT (V654A) Assay Kit is designed to measure cKIT (V654A) activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.
询价
