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背景介绍 EPHB4 or Ephrin type-B receptor 4 is a protein and member of the receptor tyrosine kinase family. It binds to its ligand, Ephrin B2, to regulate cell adhesion and migration. EPHB4 plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. It has also been implicated in prostate as well as breast cancer. 产品介绍 The EPHB4 Kinase Assay Kit is designed to measure EPHB4 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.
询价背景介绍 ADAM10 or ADAM Metallopeptidase Domain 10, is an enzyme that is involved in a diverse array of signaling pathways. It is part of the ADAM family of disintegrins and metalloproteases. It also plays a role in inflammatory disease and cancer. 产品介绍 The key to the ADAM10 Fluorogenic Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for ADAM10 reactions. A sample containing ADAM10 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=485±10 nm, λem=530±10 nm) is measured using a plate reader.
询价产品介绍 Fluorogenic Standard for the determination of 3CL protease (Main protease, Mpro) Activity. Corresponds to the Nsp5 cleavage sequence of SARS-CoV and SARS-Cov-2.
询价背景介绍 Human HER4 kinase, also known as Erb-B4, Proto-Oncogene-Like Protein C-ErbB-4, and Tyrosine Kinase-Type Cell Surface Receptor when mutated has been implicated in cancer. There has also been connection to schizophrenia with single-nucleotide polymorphisms. 产品介绍 The HER4 Assay Kit is designed to measure HER4 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
询价背景介绍
It is well established that RAS mutations are responsible more than 30% of human cancers and KRAS(G12C) is one of the KRAS mutations that is found frequently in lung and colon cancers. The G12C mutation favors the activated (GTP-bound) state of KRAS, amplifying signaling pathways that lead to oncogenesis. Recent studies discovered a small molecule called AMG510 (Amgen) that can block the KRAS(G12C)-mediated signaling pathway by locking the KRAS conformation in the GDP-bound (inactive) state. Compounds that affect the nucleotide exchange (GDP to GTP) reaction in KRAS could lead to a novel approach to the inhibition of tumor cell growth in KRAS(G12C) driven tumors.
产品介绍
The KRAS(G12C) Coupled Nucleotide Exchange Assay Kit is designed for screening and profiling of KRAS(G12C) antagonists/inhibitors by monitoring the binding of an effector protein (i.e. Ras binding domain of Raf1, RBD-cRaf) to KRAS(G12C). With this kit, a few simple steps on a microtiter plate are required for nucleotide exchange detection. First, a sample containing GDP loaded KRAS(G12C) is incubated with SOS1 and GTP for the nucleotide exchange. Next, RBD-cRAF is added and incubated for the effector-RAS binding. Then, acceptor and donor beads are added and incubated for detection followed by reading the Alpha-counts. SOS1 is a guanine nucleotide exchange factor that facilitates the exchange of GDP for GTP. GDP-loaded KRAS(G12C) is in an inactive state, and does not interact with the Ras-binding domain (RBD) of cRAF. SOS1 assists in the release GDP from KRAS(G12C) so that GTP can occupy the nucleotide binding pocket. This results in a conformational change in KRAS(G12C) that permits binding of RBD-cRAF to KRAS(G12C).
The KRAS(G12C) Coupled Nucleotide Exchange Assay Kit utilizes GST-tagged RBD-cRAF and His-tagged KRAS (G12C) to assay binding of KRAS(G12C) to RBD-cRAF in the Alpha assay. Glutathione acceptor and Ni chelate donor beads are brought into proximal range by the binding of KRAS(G12C) and RBD-cRAF, enabling the energy transfer from the donor to acceptor beads after laser excitation.
背景介绍 FYN is member of the SRC tyrosine kinase oncogene family. It plays a role in various forms of cancer and is a notable molecular target in prostate cancer. Fyn interacts with both amyloid-beta (Aβ) and tau, suggesting that inhibition of FYN has potential as a therapy for Alzheimer's disease. 产品介绍 The FYN Assay Kit is designed to measure FYN activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
询价背景介绍 EPHA2 or ephrin type-A receptor 2 is a receptor tyrosine kinase that plays a role in a number of developmental processes, as well as tissue homeostasis and cancer, including prostate cancer. It has also been seen to promote cell motility and cancer metastasis. EphA2 regulation has been linked to lens transparency, kidney repair following renal injury, bone remodeling, and inner ear development. 产品介绍 The EPHA2 Assay Kit is designed to measure EPHA2 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
询价背景介绍 ADAM17 (ADAM Metallopeptidase Domain 17) is part of the ADAM family of disintegrins and metalloproteases. Initially identified as TNF-α converting enzyme (TACE), ADAM 17 has been linked to a number of diverse signaling pathways. It cleaves ectodomains of various transmembrane proteins and regulates cytokine shedding. It also plays a role in inflammatory skin and bowel disease. 产品介绍 The ADAM17 Fluorescent Assay Kit is provided in a convenient 96-well format, with purified ADAM17, ADAM Fluorogenic Substrate, and ADAM assay buffer for 96 enzyme reactions. The key to the ADAM17 Fluorescent Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for ADAM17 reactions. A sample containing ADAM17 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=358±10 nm, λem=455±10 nm) is measured using a plate reader.
询价产品介绍 Human CD111, known as Poliovirus receptor-related 1, and nectin-1, GenBank Accession No. NM_002855, a.a. 31-355, with C-terminal Avi-Tag fused to the Fc portion of Human IgG1 and expressed in a HEK293 cell expression system. This protein runs at a higher MW by SDS-PAGE due to glycosylation. MW = 65 kDa.
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