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产品介绍 Woodchuck (Groundhog, Marmota monax) secreted programmed cell death 1 (PD-1) also known as, PDCD1, SLEB2, CD279 and HPD-L, GenBank Accession No. HQ403652.1, a.a 25-172, fused at the C-terminus to the Fc portion of Human IgG1, expressed in a HEK293 cell expression system. MW = 43 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
询价背景介绍 Isocitrate dehydrogenases are enzymes that catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) have been reported in a multitude of human cancers. While the wild type IDH2, produces α-ketoglutarate (α-KG) through the reduction of NADP+ to NADPH, the IDH2(R140Q) mutant catalyzes conversion of α-ketoglutarate to 2-hydroxy-glutarate (2-HG) by oxidizing NADPH to NADP+. Genomic studies have identified the IDH2(R140Q) mutation in various cancer types, including as glioma, chondrosarcoma, AML as well as small percentage of prostate, lung and colon cancers. 产品介绍 The IDH2(R140Q) Assay Kit is designed to measure IDH2(R140Q) activity for screening and profiling applications by measuring NADPH consumption.
询价产品介绍 Human FTO, also known as Fat mass and obesity-associated protein, ALKBH9; BMIQ14; GDFD; KIAA1752, and alpha-ketoglutarate-dependent dioxygenase, GenBank Accession No. NM_001080432, a.a 32-505 (end), with N-terminal His-Tag and expressed in a E. coli cell expression system. MW = 56 kDa.
询价产品介绍 Human Ubiquitin, also known as UBB, UBC, UBA52, and RPS27A. GenBank Accession No. NM_021009, a.a. 2-76, with C-terminal His-tag, expressed in an Sf9 expression system.
询价产品介绍 Mouse secreted Programmed Cell Death 1 (PD-1)-Fc fusion protein, also known as CD279, PDCD1, and SLEB2, GenBank Accession No. NM_008798, a.a. 25-167, with a C-terminal Avi-His-tag, expressed in a HEK293 cell expression system. MW = 19 kDa. This protein runs at an apparent higher molecular weight due to glycosylation.
询价背景介绍 CD38, a differentiation antigen of B lymphocytes, is a type II integral membrane protein. It is also known as ADP-ribosyl cyclase and nicotinamide adenine dinucleotide (NAD) glycohydrolase. Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in various cells, including pancreatic β cells. The major enzymatic activity of CD38 is the hydrolysis of NAD. CD38 is a prognostic biomarker for acute B lymphoblastic leukemia. 产品介绍 The CD38 Inhibitor Screening Assay Kit (Hydrolase Activity) is designed to measure the glycohydrolase activity of CD38 for screening and profiling applications. The CD38 assay kit comes in a convenient 96-well format, with purified recombinant CD38 enzyme, its substrate N6-etheno-NAD (ε-NAD), and CD38 assay buffer for 100 enzyme reactions. In addition, the kit includes the CD38 inhibitor apigenin for use as a control inhibitor.
询价背景介绍 Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE4D has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP. Inhibition of PDE4D activity by its inhibitors leads to an elevated intracellular level of cAMP. The PDE4D gene encodes at least 9 different isoforms, and has been linked to stroke, asthma, arrhythmia, and cardiac myopathy, making it an important therapeutic target. 产品介绍 The PDE4D Transient Pack is designed to provide the tools necessary for transiently transfecting and for screening inhibitors of PDE4D7 in cultured HEK293 cells. The assay is based on transfecting cells with the CRE luciferase reporter. CRE reporter contains the firefly luciferase gene under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates CRE binding protein (CREB) to bind CRE and induce the expression of luciferase. Forskolin is commonly used to raise the intracellular level of cAMP in cell physiology studies. When cells transiently transfected with CRE reporter are activated by forskolin, the intracellular level of cAMP is upregulated, which induces the expression of CRE luciferase reporter. However, when cells are co-transfected with PDE4D7 expression vector and CRE reporter, the level of forskolin-induced cAMP is reduced, resulting in lower expression level of luciferase. When cells are treated with PDE4D inhibitor to inhibit PDE4D7 activity, cAMP level is restored, resulting in higher luciferase activity. The PDE4D Transient Pack contains transfection-ready CRE luciferase reporter. This reporter contains transfection-ready vectors for PDE4D7 expression and a CRE vector containing firefly luciferase as a cAMP pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the PDE4D Cell-Based Activity Reporter Detection Valuepack is the CRE luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized cAMP response elements (CRE) located upstream of a minimal promoter. The vector is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency. The pack also includes the PDE4D7 expression vector, and the adenylate cyclase activator, forskolin. Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 and HeLa cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. *Note: the kit may be used with other cell lines than HEK293 or HeLa, but an alternate cell culture medium may be required for optimal cell growth
询价背景介绍 The Notch signaling pathway controls cell fate decisions in vertebrate and invertebrate tissues. NOTCH signaling is triggered through the binding of a transmembrane ligand to Notch transmembrane receptor (NOTCH1/ NOTCH2/NOTCH3/NOTCH4) on a neighboring cell. This results in proteolytic cleavage of the NOTCH receptor, releasing the constitutively active intracellular domain of NOTCH (NICD). NICD translocates to the nucleus and associates with transcription factors CSL (CBF1/RBPJκ/Suppressor of Hairless/Lag-1) and coactivator Mastermind to turn on transcription of Notch-responsive genes. 产品介绍 The Notch1/CSL Transient Packis designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Notch signaling pathway in HEK293 cultured cells. The kit contains transfection-ready vectors containing firefly luciferase as a Notch pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HEK293 cells. The key to the Notch1/CSL Transient Pack is the expression vector for NOTCH1 that has a deletion of the entire extracellular domain (Notch1DE). Inside the cells, the NOTCH1 DE can be cleaved by γ-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency. The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity. Additionally, the pack includes cell culture medium (BPS Medium 1) that has been optimized for use with HEK293 cells*. BPS Medium 1 includes MEM medium, 10% fetal bovine serum, 1% non-essential amino acids, sodium pyruvate, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These luciferase reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. *Note: the kit may be used with other cell lines than HEK293, but an alternate cell culture medium may be required for optimal cell growth,
询价背景介绍 The Myc signaling pathway plays an important role in cell proliferation, differentiation, transformation and apoptosis. The c-Myc protein is a transcription factor that heterodimerizes with Max to regulate Myc signaling pathway responsive genes. Myc mutations have been linked to the development of a number of human cancers, including Burkitt's lymphoma, cervical, ovarian, breast, lung and pancreatic carcinoma, making Myc a promising therapeutic target for cancer treatment. 产品介绍 The Myc Transient Pack is designed to provide the tools necessary for transiently transfecting and monitoring the activity of the Myc signaling pathway in cultured HCT116 cells. The Myc Transient Pack contains transfection-ready vectors containing firefly luciferase as a Myc pathway-responsive reporter and constitutively expressing Renilla luciferase as a transfection control. It also includes the Dual Luciferase detection reagents to detect both luciferase activities and specialized medium for growing and assaying HCT116 cells. .The key to the Myc Transient Pack is the expression vectors for c-Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a minimal promoter. The Myc reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency. The pack also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. This negative control is critical for determining pathway-specific effects and background luciferase activity. An "empty" expression vector without the c-Myc gene is also provided as an additional negative control. Additionally, the pack includes cell culture medium that has been optimized for use with HCT116 cells*. HCT116 is a human colon cancer cell line with a mutated β -catenin which leads to the accumulation of β-catenin and constitutive activation of the Myc signaling pathway. This medium includes 10% fetal bovine serum, and 1% Pen/Strep. Finally, the pack provides the Dual Luciferase (Firefly-Renilla) Assay System. These reagents provide highly sensitive, stable detection of firefly luciferase activity and Renilla luciferase activity. The dual luciferase reagents can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required. *Note: the kit may be used with other cell lines than HCT116, but an alternate cell culture medium may be required for optimal cell growth. For HEK293 cells, we recommend using BPS Medium 1 (BPS Bioscience, #79259).
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