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产品介绍 Human Death-Associated Protein Kinase-1 (DAPK1) (GenBank Accession No. NM_004938.2), a.a. 2-363, with N-terminal GST-tag, MW = 67 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
询价背景介绍 COT is an oncogene that can activate both the MAP kinase and JNK kinase pathways. COT activates IkappaB kinases and induces the nuclear production of NF-κB. C-terminal catalytic domain of KSR2 associates with COT and KSR2 can negatively regulates the kinase activity of COT in vitro. Co-transfection of KSR2 with COT in cells lead to reduced COT-mediated ERK activation and COT-induced IL8 production in a dose-dependent manner. Cot is one of the MAP kinase kinase kinases that regulates the ERK1/ERK2 pathway in response to IL-1. Blockage of expression of Cot results in failure of IL-1 to induce an increase in IL-8 and MIP-1betamRNA levels. 产品介绍 Human Cot/Tpl2 (GenBank Accession No. NM_005204), (a.a. 30-397), with N-terminal GST-His-tag, MW= 72 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
询价产品介绍 Human Checkpoint kinase 2 (GenBank Accession No. NM_007194), full length, with N-terminal His-tag. MW=91 kDa. Expressed in a Baculovirus infected Sf9 cell expression system.
询价背景介绍 PLK3 or Polo-like kinase 3 is a cytokine-inducible kinase that is a member of the Polo family of serine/threonine protein kinases. PLK3 contains both a catalytic domain and a putative regulatory domain and plays an important role in the regulation of cell cycle progression and tumorigenesis. PLK3 is closely associated with centrosomes in several human cell lines and its localization is dependent on the integrity of microtubules. Hence, PLK3 is also involved in regulating microtubule dynamics and centrosomal function. 产品介绍 Human Plk3 (GenBank Accession No. NM_004073), (a.a. 58-339) with N-terminal His-tag, MW=36.7 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Source: Baculovirus infected Sf9 cells Purity:≥80% by SDS-PAGE. Specific Activity: 500 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP.
询价背景介绍 PLK2 or Polo-like kinase 2 is a serum-inducible kinase that is a member of the Polo family of serine/threonine protein kinases which play an essential role in normal cell division. PLK2 phosphorylates the P4.1-associated protein (CPAP), a human homologue of SAS-4, in procentriole formation during the centrosome cycle. PLK2 may also play a role as a tumor suppressor since it interacts with TSC1 protein which in turn inhibits mTOR signaling, tumor growth and chemosensitivity under hypoxic conditions. Human lung tumor cells deficient in PLK2 grow larger than control tumors, and PLK2 interacts with endogenous TSC1 protein in these tumors. 产品介绍 Human Plk2 (GenBank Accession No. NM_006622), full length, with N-terminal GST-tag, expressed in a baculovirus infected Sf9 cell expression system. Theoretical MW is 107 kDa.
询价背景介绍 PLK1 is a member of the Polo-Like Kinase family that localize to centrosomes or spindle pole bodies and undergo dramatic subcellular relocation during the cell cycle. Deregulated activities of PLKs often result in abnormalities in centrosome duplication, maturation, and/or microtubule dynamics. PLKs also regulate the function of the Golgi complex. Deregulated expression of human PLK1 is strongly correlated with the development of many types of malignancies, and ectopic expression of PLK1 dominant negative protein leads to rapid cell death. 产品介绍 Human Plk1 (GenBank Accession No. NM_005030), full length with C-terminal His-tag, MW=68 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
询价背景介绍 AMPK is a heterotrimer protein kinase consisting of an α catalytic subunit, and non-catalytic β and γ subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status (1). In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol (2). 产品介绍 Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. The gene accession number for the three subunits (A1/B1/G1) is NM_006251, NM_006253, and NM_002733, respectively. This protein has been activated with CAMKK.
询价背景介绍 ALK 2 is a receptor serine/threonine kinase that is member of the ALK family and is upstream of signaling pathway involving the SMAD proteins especially SMAD1/5/8. Knockdown of ALK2, but not TGFβRI (ALK5), abrogates endoglin-mediated decrease in cell motility of prostate cancer cells and constitutively active ALK2 is sufficient to restore a low-motility phenotype in endoglin deficient cells (1). Therefore, endoglin decreases prostate cancer cell motility through activation of the ALK2-Smad1 pathway. ALK2 is the key gene involved in Fibrodysplasia ossificans progressiva (FOP), a rare autosomal dominant congenital disorder characterized by progressive heterotopic bone formation in muscle tissues (2). 产品介绍 Recombinant human activin receptor-like kinase 2, or ALK2 (a.a. 147-end), with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system. The gene accession number is NM_001105.
询价背景介绍 MST1 belongs to a family of proteins that share similarity with a budding yeast serine/threonine kinase, sterile-20 (Ste20). Endogenous full-length MST1 is activated by a variety of stressful stimuli, accompanied by the secondary appearance of a 36 kDa Thr183-phosphorylated, caspase-cleaved catalytic fragment. Recombinant MST1 undergo a robust autoactivation in vitro, mediated by an intramolecular autophosphorylation on the activation loop of an MST dimmer. MST1 can initiate apoptosis when transiently overexpressed in mammalian cells. Interference with the ability of endogenous MST1 to associate with the putative tumor suppressor proteins Nore1/RASSF can inhibit Ras-induced apoptosis. 产品介绍 Human STK4 (serine/threonine kinase 4), also known as MST-1 (Mammalian STE20-like protein kinase 1) or KRS2, GenBank Accession No. NM_006282, a.a. 2-488(end), with N-terminal GST-tag, MW=81.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
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