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产品介绍 HDAC assay buffer is an essential reagent for signal generation in HDAC assays performed with any of BPS Bioscience's HDAC fluorimetric substrates or assay kits. An appropriate fluorimetric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.) in HDAC assay buffer. Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Trichostatin A is included asan 'inhibitor stop' for class I and II HDACs.
询价产品介绍 HDAC assay developer is an essential reagent for signal generation in HDAC assays performed with any of BPS Bioscience's HDAC fluorimetric substrates or assay kits. An appropriate fluorimetric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Trichostatin A is included as an 'inhibitor stop' for class I and II HDACs.
询价背景介绍 Sirtuins are NAD+ dependent class III histone deacetylases that regulate important biological processes including metabolism and aging. In human, there are seven isoforms of Sirtuins, SIRT1 to SIRT7. Four of the isoforms, SIRT4 to SIRT7, have no detectable or very weak deacetylase activity. Sirtuin 6 (SIRT6) is shown to be a potent defatty-acylase of lysine residues. 产品介绍 The Sirtuin 6 Fluorogenic Assay Kit is a complete assay system designed to measure SIRT6 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT6 activity measurements. In addition, the kit includes purified SIRT6 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT6 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT6 activity level. First, the Sirtuin 6 fluorometric substrate, containing a myristoylated lysine side chain, is incubated with purified SIRT6 enzyme. The demyristoylation sensitizes the substrate so subsequent treatment with the SIRT Developer produces a fluorophore that can then be measured using a fluorescence reader.
询价产品介绍 Human HDAC11 (full length) (GenBank Accession No. NM_024827) untagged, MW= 39.6 kDa, expressed in baculovirus expression system.
询价背景介绍 NcoR2 , nuclear receptor corepressor, are transcriptional corepressors that play an essential role in the regulation of development and metabolism. The interaction of HDAC3 with the deacetylase activation domain , DAD, of NCOR2/SMRT is required for activation of the otherwise inert deacetylase. 产品介绍 Human NCOR2/SMRT, DAD domain, a.a. 395-489, GenBank Accession No. NM_006312, N-terminal GST-tag, MW= 39 kDa, expressed in E. coli system. Source: E. coli. Purity: > 60% by SDS-PAGE.
询价背景介绍 SETD7 or SET domain containing lysine methyltransferase 7 is a lysine methyltransferase which can methylates lysine-4 (K4) in histone H3 in vitro and in vivo. Methylation of K4 in histone H3 by SETD7 and methylation of K9 in histone H3 by SUV39H1 were found to have differential effects on subsequent histone acetylation by p300. SETD7 can also methylate p53 at lys372 within the C-terminal regulatory region. Methylated p53 is restricted to the nucleus and the modification positively affects its stability. SETD7 regulates the expression of p53 target genes in a manner dependent on the p53 methylation site. 产品介绍 Human recombinant SET domain containing lysine methyltransferase 7 (SETD7), or SET7/9 (GenBank Accession No. NM_030648), a.a. 52-266 (end) with N-terminal His-GST-tag, MW = 63 kDa, expressed in a baculovirus infected Sf9 cell expression system.
询价产品介绍 Human Sirtuin 7 (GenBank Accession No. NM_016538), full length with C-terminal FLAG-tag, MW= 46 kDa, expressed in an E. coli expression system.
询价产品介绍 Human Sirtuin 6 (GenBank Accession No. NM_016539), full length with N-terminal GST-tag, MW= 65 kDa, expressed in an E. coli expression system.
询价产品介绍 Human Sirtuin 5 (GenBank Accession No. NM_012241), full length with N-terminal GST-tag, MW= 59.8 kDa, expressed in an E. coli expression system.
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