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产品介绍 Malarial (Plasmodium falciparum) HDAC, Genbank Accession No. AAD22407.1, full length (1-450) with C-terminal His-tag and FLAG-tag, MW=53.4 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
询价背景介绍 HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. Fluorescence is therefore directly related to HDAC activity. 产品介绍 The Fluorogenic HDAC2 Assay Kit is a complete assay system designed to measure histone deacetylase 2 (HDAC2) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC2 activity measurements. In addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC2 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC2. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.
询价背景介绍 HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. Fluorescence is therefore directly related to HDAC activity. 产品介绍 The Fluorogenic HDAC1 Assay Kit is a complete assay system designed to measure histone deacetylase 1 (HDAC1) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC1 activity measurements. In addition, the kit includes purified HDAC1 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC1 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC1. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.
询价产品介绍 Human HDAC10 (a.a. 2-631) (GenBank Accession No. NM_032019) with N-terminal FLAG-tag, MW= 67 kDa, expressed in Sf9 cells via a baculovirus expression system.
询价产品介绍 Mouse HDAC1, GenBank Accession No. NM_008228, amino acids 1-482 (full length), with C-terminal His-tag and C-terminal FLAG-tag, MW = 57 kDa, expressed in an Sf9 baculovirus expression system. Recombinant HDAC1 forms a complex with endogenous Hsp70. The identity of Hsp70 was confirmed by MALDI-TOF mass spectrometry.
询价产品介绍 Mouse HDAC6, also known as histone deacetylase 6, GenBank Accession No. NM_010413, a.a. 2-1149 (end) with N-terminal GST-Tag, expressed in baculovirus expression system. MW = 152 kDa.
询价产品介绍 Human HDAC6 (GenBank Accession No. NM_006044), full length with C-terminal FLAG-tag, MW= 132 kDa, expressed in baculovirus expression system.
询价产品介绍 Human HDAC2 , GenBank Accession No. Q92769, full length with C-terminal FLAG-tag, MW= 60 kDa, expressed in baculovirus expression system.
询价产品介绍 Human HDAC1, GenBank Accession No. NM_004964, full length with C-terminal FLAG and C-terminal His-tag, MW= 56 kDa, expressed in baculovirus expression system.
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