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背景介绍 EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4. 产品介绍 Human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end with N-terminal His-GST-tag, MW= 114 kDa, expressed in a Sf9 baculovirus expression system. This protein is inactive alone, although it is functional if combined with other proteins in the complex.
询价背景介绍 Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. 产品介绍 Recombinant Jellyfish (Aequorea victoria) green fluorescent protein, full length with C-terminal His-tag, MW= 29.9 kDa, expressed in an E. coli expression system. GFP absorbs light at 395 and 470 nm, emits green fluorescence at 509 nm.
询价产品介绍 Human recombinant CoREST (GenBank Accession No. NM_015156), a.a. 305-end, with N-terminal His-tag, MW=20 kDa, expressed in an E. coli expression system.
询价背景介绍 BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform (Bcl-xL) acts as an apoptotic inhibitor and the shorter form (Bcl-xS) acts as an apoptotic activator. 产品介绍 Human BCL-XL (GenBank Accession No. Z23115), a.a. 1-212 with C-terminal His-tag, MW = 28 kDa, expressed in an E. coli expression system.
询价背景介绍 Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). 产品介绍 Human Bcl-2 (GenBank Accession No. P10415), a.a. 1-211 with C-terminal His-tag, MW = 27 kDa, expressed in an E. coli expression system.
询价产品介绍 Human ALAS2 (aminolevulinate, delta-, synthase 2) (GenBank Accession No. NM_000032), (a.a. 136-553) with N-terminal His-tag, MW = 46 kDa, expressed in an E. coli expression system.
询价产品介绍 The HSP90β_x0001_ (C-Terminal) TR-FRET assay kit is designed to measure the inhibition of HSP90α binding to its protein target PPID (also known as Cyclophilin D or Cyp40) in a homogeneous, 384 reaction format. This assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple. A sample containing terbium-labeled donor, dye-labeled acceptor, HSP90β, substrate, and an inhibitor is incubated for 2 hours. The protein-protein interaction is then assayed by measuring the TR-FRET between the PPID and HSP90β using a fluorescence reader.
询价产品介绍 The HSP90α (C-Terminal Domain) TR-FRET assay kit is designed to measure the inhibition of HSP90α binding to its protein target PPID (also known as Cyclophilin D or Cyp40) in a homogeneous, 384 reaction format. This assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple. A sample containing terbium-labeled donor, dye-labeled acceptor, HSP90α, substrate, and an inhibitor is incubated for 2 hours. The protein-protein interaction is then assayed by measuring the TR-FRET between the PPID and HSP90α using a fluorescence reader.
询价产品介绍 Human AHCY (human S-adenosyl homocysteine hydrolase), GenBank Accession No. NM_000687, (a.a. 2-end) with N-terminal GST-tag, MW = 73 kDa, expressed in an E. coli expression system.
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