服务平台拥有丰富的技术服务经验，一流的仪器设备，先进的检测手段和严格的质量控制体系，力求为全球药物研发机构提供优质高效的技术服务
背景介绍 L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion. 产品介绍 The IDO1 Inhibitor Screening Assay Kit is designed to measure IDO1 enzyme inhibition. The IDO1 Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at λ=321 nm.
询价背景介绍 The nuclear factor erythroid 2-related factor (Nrf2) is a transcription factor that induces the expression of antioxidant and phase II proteins by binding to the ARE (antioxidant response element) region of the gene promoter. Under basal conditions, Nrf2 is retained in the cytosol by binding to the cytoskeletal protein Keap1. Upon exposure to oxidative stress and other ARE activators, Nrf2 is released from Keap1. It then translocates to the nucleus, where it can bind to the ARE and activate expression of antioxidant and phase II proteins that protect the cell from oxidative damage. Because Nrf2 activation leads to a coordinated antioxidant and antiinflammatory response, and Keap1 represses Nrf2 activation, Keap1 has become a very attractive drug target. 产品介绍 The Keap1:Nrf2 Inhibitor Screening Assay Kit is designed for identification of inhibitors of Keap1:Nrf2 binding using fluorescence polarization. Using this kit, only one simple step on a microtiter plate is required for Keap1:Nrf2 inhibitor screening reactions. The Keap1 protein and fluorescently labeled Nrf2 peptide are incubated with a sample containing testing inhibitors for 30 minutes. The change in fluorescent polarization can then be measured using a fluorescence reader.
询价产品介绍 Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD- 1[Biotinylated]:PD-L2 Inhibitor Screening Colorimetric Assay Kit is designed for screening inhibitors of PD-1 receptor binding to PD-L2 ligand. The key to this kit is the high affinity of biotin-labeled PD-1 for streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L2 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L2 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
询价产品介绍 Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD- 1[Biotinylated]:PD-L1 Inhibitor Colorimetric Screening Assay Kit is designed for screening inhibitors of PD-1 receptor binding to PD-L1 ligand. This kit comes in a convenient 96-well format, with biotin-labeled PD-1, purified PD-L1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high affinity of biotin-labeled PD-1 for streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
询价产品介绍 Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD-1:PDL2[ Biotinylated] Inhibitor Screening Colorimetric Assay Kit is designed for screening inhibitors of PD-L2 ligand binding to PD-1 receptor. The key to this kit is the high affinity of biotin-labeled PD-L2 for streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated on a 96-well plate. Next, PD-L2 is incubated with PD-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
询价产品介绍 Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD-1:PDL1[Biotinylated] Inhibitor Screening Colorimetric Assay Kit is designed for screening inhibitors of PD-L1 ligand binding to PD-1 receptor. The key to this kit is the high affinity of biotin-labeled PD-L1 for streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated on a 96-well plate. Next, PD-L1 is incubated with PD-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
询价产品介绍 The PD-1:PD-L2 Homogeneous Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L2. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing PD-1 and an inhibitor of choice is incubated with the biotinylated PD-L2 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
询价产品介绍 The PD-1:PD-L1 Homogeneous Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L1. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing PD-1 and an inhibitor of choice is incubated with the biotinylated PD-L1 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
询价背景介绍 Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. 产品介绍 The PD-1:PD-L2 TR-FRET Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L2 in a homogeneous 384 reaction format. Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing europium-labeled (Eu) PD-1, dye-labeled acceptor, biotin-labeled PD-L2, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
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