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背景介绍 CD226 is an activating receptor expressed on the surface of natural killer (NK) cells, monocytes, platelets, and T cells. Its natural ligands are CD112 and CD155 which are expressed by antigen presenting cells (APC). Binding of CD226 to either CD112 or CD155 triggers NK cell effector function and can promote Th1 cell differentiation in T cells. CD226 competes for binding with CD112 and CD155 with the co-inhibitory receptor TIGIT. Agonistic CD226 monoclonal antibodies are being explored as potential therapeutics in settings where immune activation is beneficial, such as cancer. 产品介绍 The CD226:CD155 Homogeneous Assay Kit is designed to measure the inhibition of CD226 binding to CD155 (PVR). The CD226:CD155 Homogeneous Assay Kit comes in a convenient AlphaLISA® format with purified biotinylated CD226, His-tagged CD155, and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing CD226 and an inhibitor of choice is incubated with the CD155 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
询价背景介绍 CD226 is an activating receptor expressed on the surface of natural killer (NK) cells, monocytes, platelets, and T cells. Its natural ligands are CD112 and CD155 which are expressed by antigen presenting cells (APC). Binding of CD226 to either CD112 or CD155 triggers NK cell effector function and can promote Th1 cell differentiation in T cells. CD226 competes for binding with CD112 and CD155 with the co-inhibitory receptor TIGIT. Agonistic CD226 monoclonal antibodies are being explored as potential therapeutics in settings where immune activation is beneficial, such as cancer. 产品介绍 The CD226:CD112 Homogeneous Assay Kit is designed to measure the inhibition of CD226 binding to CD112 (PVRL2). The CD226:CD112 Homogeneous Assay Kit comes in a convenient AlphaLISA® format with purified biotinylated CD226, His-tagged CD112, and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing CD226 and an inhibitor of choice is incubated with the CD112 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
询价背景介绍 L-Trp is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 &IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion. Inhibitors of IDO1 can bind either irreversibly (covalent binding to the protein) or reversibly (non-covalent association) to the enzyme. The IDO1 Inhibitor Mechanism of Action Assay Kit allows researchers to determine the mechanism of IDO1 inhibitor binding. At high concentrations, e.g., 10x the IC50 concentration of the inhibitor, both reversible and irreversible inhibitors will interfere with IDO1 activity. However, after sufficient dilution, e.g. to 0.3x the IC50 concentration, reversible inhibitors will dissociate, relieving inhibition of the IDO1 enzyme. Irreversible inhibitors will not dissociate, and will continue to inhibit the IDO1 enzyme at the same level. Therefore, by monitoring enzymatic activity after inhibitor dilution, the mechanism of binding to the IDO1 protein can be determined. 产品介绍 The IDO1 Inhibitor Mechanism of Action (MoA) Assay Kit is designed to determine the mechanism of IDO1 enzyme inhibition (e.g., reversible or irreversible inhibition). The IDO1 Mechanism of Action Assay Kit is simple to use. Inhibitor and enzyme are preincubated allowing the inhibitor to bind. After preincubation, the mixture is diluted into reaction buffer containing the L-tryptophan (L-Trp) substrate and all necessary coupled reaction components. This allows reversible inhibitors to dissociate, while irreversible inhibitors remain associated with the IDO1 enzyme. IDO1 activity is determined by measuring the absorption of the reaction product at _x0001_=320 - 325 nm.
询价背景介绍 L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Tryptophan 2,3 dioxygenase (TDO), a rate limiting enzymes in this pathway, is upregulated in many tumors, providing cancer cells with an avenue for immune evasion. 产品介绍 The TDO Fluorogenic Inhibitor Screening Assay Kit is designed to measure enzyme inhibition of tryptophan 2,3-dioxygenase (TDO). The TDO Fluorogenic Inhibitor Screening Assay Kit is simple to use and detects fluorescence at long wavelengths, which minimizes potential errors due to compound interference. In the assay, the inhibitor and enzyme are added to a sample containing L-Trp substrate. After a 1 hour incubation at room temperature, the fluorescence solution is added and incubated at 37°C for four hours. Activity is measured by reading sample fluorescence at λ=510 nm following excitation of the reaction product at λ=400 nm.
询价背景介绍 L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion. 产品介绍 The IDO1 Fluorogenic Inhibitor Screening Assay Kit is designed to measure enzyme inhibition of indoleamine 2,3-dioxygenase 1 (IDO1). The IDO1 Fluorogenic Inhibitor Screening Assay Kit is simple to use and detects fluorescence at long wavelengths, which minimizes potential errors due to compound interference. In the assay, the inhibitor and enzyme are added to a sample containing L-Trp substrate. After a 1 hour incubation at room temperature, the fluorescence solution is added and incubated at 37°C for four hours. Activity is measured by reading sample fluorescence at λ=510 nm following excitation of the reaction product at λ=400 nm.
询价背景介绍 OX40 (CD134) is a co-stimulatory receptor expressed on the surface of T cells. Binding of OX40 to its ligand, OX40L (CD252), present on dendritic cells, potentiates T cell activation and T cell effector function. Studies have shown that OX40 agonists can increase anti-tumor immunity and improve tumor-free survival in pre-clinical studies. Alternatively, OX40 antagonists offer potential as therapeutics for inflammatory diseases. 产品介绍 The OX40[Biotinylated]:OX40L Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of OX40:OX40L interaction. The key to this kit is the high sensitivity of detection of biotinlabeled OX40 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, OX40L is coated on a 96-well plate. Next, OX40-biotin is incubated with OX40L on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
询价背景介绍 Signal-regulatory protein alpha (SIRP-α), also known as CD172a, is a cell surface protein expressed mainly by myeloid cells such as macrophages. Its receptor, CD47, is ubiquitously expressed on the surface of all cells and has been found to be overexpressed in some cancers. CD47 binding to SIRP-α delivers a "Do Not Eat Me" signal to macrophages. In the context of a tumor, this signaling aids in tumor evasion of the immune system. Inhibitors of this interaction hold great promise for cancer immunotherapy. 产品介绍 The CD47:SIRP-α_x0001_[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD47:SIRP-α interaction. The key to this kit is the high sensitivity of detection of biotinlabeled SIRP-α by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD47 is coated on a 96-well plate. Next, SIRP-α-biotin is incubated with CD47 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
询价背景介绍 L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion. 产品介绍 The IDO2 Inhibitor Screening Assay Kit is designed to measure mouse IDO2 enzyme inhibition. The Mouse IDO2 Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at l=320 - 325 nm. BACKGROUND: L-tryptophan
询价背景介绍 L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion. 产品介绍 The Mouse IDO1 Inhibitor Screening Assay Kit is designed to measure inhibition of the murine IDO1 enzyme. This kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate. After a room temperature incubation, activity is determined by measuring the absorption of reaction product at l=320-325 nm.
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