Cathepsin V Inhibitor Screening Assay Kit

背景介绍 Cathepsin V, also called Cathepsin L2, is a lysosomal cysteine endopeptidase with high sequence homology to cathepsin L and other members of the papain superfamily of cysteine proteinases. Its expression is regulated in a tissue-specific manner and is high in thymus, testis and cornea. Expression analysis of cathepsin V in human tumors revealed widespread expression in colorectal and breast carcinomas, suggesting a possible role in tumor processes. 产品介绍 The Cathepsin V Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin V for screening and profiling applications. The Cathepsin V assay kit comes in a convenient 96-well format, with purified Cathepsin V, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions. In addition, the kit includes the cathepsin inhibitor E64 for use as a control inhibitor.

Cathepsin S Inhibitor Screening Assay Kit

背景介绍 Cathepsin S is a cysteine protease of the papain family, found inside the lysosomal/endosomal compartments of antigen-presenting cells such as B cells, macrophages and dendritic cells. Cathepsin S participates in the degradation of antigenic proteins to peptides for presentation to the MHC class II. Cathepsin S has additional biological effects, acting as elastase on the surface of vascular endothelial cells, a process that contributes to microvascular complications in diabetes mellitus. 产品介绍 The Cathepsin S Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin S for screening and profiling applications. The Cathepsin S assay kit comes in a convenient 96-well format, with purified Cathepsin S, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions. In addition, the kit includes the cathepsin inhibitor E64 for use as a control inhibitor.

BCL2L2 TR-FRET Assay Kit

产品介绍 The BCL2L2 TR-FRET Assay Kit is designed to measure the inhibition of BCL2L2 (BCL-w) binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BCL2L2, peptide ligand, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.

ABL (T315I) Assay Kit

产品介绍 The Abelson (ABL) family of nonreceptor tyrosine kinases is critical to the progression and treatment of chronic myeloid leukemia (CML). The single-point mutation T315I has been linked to drug resistance and poor prognosis, making it an important drug target. The ABL (T315I) Assay Kit is designed to measure ABL activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The ABL (T315I) Assay Kit comes in a convenient 96-well format, with enough purified recombinant ABL enzyme, ABLtide peptide, ATP, and kinase assay buffer for 100 enzyme reactions.

CBL-B TR-FRET Assay Kit

产品介绍 Human Casitas B-lineage lymphoma proto-oncogene b (CBL-B) is an E3 ubiquitinprotein ligase that functions as a negative regulator of T-cell activation. It is a promising drug target in cancer immunotherapy. The CBL-B TR-FRET Assay Kit is designed to measure CBL-B auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes biotin-labeled Ubiquitin and a terbium-labeled anti-CBL-B antibody to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.

PDE4B3 Assay Kit

产品介绍 Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4 selective inhibitors are currently in clinical trials for the treatment of diseases related to inflammatory disorders. PDE4B3 mRNA is readily expressed in both oligodendrocytes and neurons and it is the first cAMP-specific phosphodiesterase to be associated with hippocampal long-term potentiation (LTP), the most prominent cellular model for learning and memory formation. The PDE4B3 Assay Kit is designed for identification of inhibitors of PDE4B3 using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4B3 to the binding agent. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light. The PDE4B3 Assay Kit comes in a convenient 96-well format, with purified PDE4B3 enzyme, fluorescently labeled PDE4B3 substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE4B3 Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4B3 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4B3 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

PDE4B (Dog) Assay Kit

产品介绍 Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4 selective inhibitors are currently in clinical trials for the treatment of diseases related to inflammatory disorders. Altered activity of PDE4B has been associated with schizophrenia and bipolar disorder. It has also recently been found to modulate cognition, as reduction in PDE4B activity improves memory and long-term plasticity in mouse models. The dog PDE4B (dPDE4B) Assay Kit is designed for identification of inhibitors of dog PDE4b using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by canine PDE4B to the binding agent. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light. The Dog PDE4B Assay Kit comes in a convenient 96-well format, with purified dPDE4B enzyme, fluorescently labeled dPDE4B substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the Dog PDE4B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for dPDE4B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing dPDE4B for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

Rat PDE4B Assay Kit

产品介绍 Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4 selective inhibitors are currently in clinical trials for the treatment of diseases related to inflammatory disorders. Altered activity of PDE4B has been associated with schizophrenia and bipolar disorder. It has also recently been found to modulate cognition, as reduction in PDE4B activity improves memory and long-term plasticity in mouse models. The Rat PDE4B Assay Kit is designed for identification of inhibitors of rat PDE4B (rPDE4B) using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by rPDE4B to the binding agent.

Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light. The Rat PDE4B Assay Kit comes in a convenient 96-well format, with purified rPDE4B enzyme, fluorescently labeled rPDE4B substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the Rat PDE4B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for rPDE4B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing rPDE4B for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

c-Raf Kinase Assay Kit

产品介绍 c-Raf (Raf-1; proto-oncogen c-Raf) is a member of the Raf (rapidly accelerated fibrosarcoma) kinase family that plays an important role in regulating the MEK and ERK pathways. The c-Raf Kinase Assay Kit is designed for measuring c-Raf activity for screening and profiling applications. The c-Raf Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant c-Raf enzyme, c-Raf substrate, ATP and kinase assay buffer for 100 enzyme reactions.