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背景介绍
Luciferase is the general term given to a class of oxidative enzymes that catalyze reactions that give off light, a process known as bioluminescence (Fig. 1). In biology, researchers can take advantage of this reaction and use it as a readout for various biological processes. This has perhaps been exploited most in luciferase reporter cell lines where a promoter region from a gene of interest is placed immediately upstream of the coding sequence for luciferase. In this system, transcriptional activation of the gene of interest leads to a level of luciferase expression that is proportional to the level of gene activation. The level of activation is then assessed by lysing the cells and adding the luciferase substrate, D-luciferin. Using the One-Step Luciferase Assay System from BPS Bioscience, this is accomplished in a single step as the lysis buffer has been formulated with D-luciferin. After a brief incubation period, bioluminescence is read on a luminometer.
产品介绍
The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features:
• Sensitive - highly sensitive detection of firefly luciferase activity.
• Stable - the signal output is stable for more than two hours, providing flexibility with regard to incubation time
• Convenient - simple one-step, homogeneous protocol.
• High-throughput - one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications
• Compatibility - works well with a variety of common media containing 0-10% serum and phenol red.
• Instrumentation - does not require a luminometer with injectors.
背景介绍
Luciferase is the general term given to a class of oxidative enzymes that catalyze reactions that give off light, a process known as bioluminescence (Fig. 1). In biology, researchers can take advantage of this reaction and use it as a readout for various biological processes. This has perhaps been exploited most in luciferase reporter cell lines where a promoter region from a gene of interest is placed immediately upstream of the coding sequence for luciferase. In this system, transcriptional activation of the gene of interest leads to a level of luciferase expression that is proportional to the level of gene activation.
The level of activation is then assessed by lysing the cells and adding the luciferase substrate, D-luciferin. Using the One-Step Luciferase Assay System from BPS Bioscience, this is accomplished in a single step as the lysis buffer has been formulated with D-luciferin. After a brief incubation period, bioluminescence is read on a luminometer.
产品介绍
The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features:
• Sensitive - highly sensitive detection of firefly luciferase activity.
• Stable - the signal output is stable for more than two hours, providing flexibility with regard to incubation time
• Convenient - simple one-step, homogeneous protocol.
• High-throughput - one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications
• Compatibility - works well with a variety of common media containing 0-10% serum and phenol red.
• Instrumentation - does not require a luminometer with injectors.
背景介绍
Luciferase is the general term given to a class of oxidative enzymes that catalyze reactions that give off light, a process known as bioluminescence (Fig. 1). In biology, researchers can take advantage of this reaction and use it as a readout for various biological processes. This has perhaps been exploited most in luciferase reporter cell lines where a promoter region from a gene of interest is placed immediately upstream of the coding sequence for luciferase. In this system, transcriptional activation of the gene of interest leads to a level of luciferase expression that is proportional to the level of gene activation.
The level of activation is then assessed by lysing the cells and adding the luciferase substrate, D-luciferin. Using the One-Step Luciferase Assay System from BPS Bioscience, this is accomplished in a single step as the lysis buffer has been formulated with D-luciferin. After a brief incubation period, bioluminescence is read on a luminometer.
产品介绍
The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features:
• Sensitive - highly sensitive detection of firefly luciferase activity.
• Stable - the signal output is stable for more than two hours, providing flexibility with regard to incubation time
• Convenient - simple one-step, homogeneous protocol.
• High-throughput - one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications
• Compatibility - works well with a variety of common media containing 0-10% serum and phenol red.
• Instrumentation - does not require a luminometer with injectors.
背景介绍 Firefly luciferase has been used as a sensitive reporter to study a wide range of biological responses. However the change of the expression of Firefly luciferase reporter can be due to a global effect instead of a specific effect. The accuracy of Firefly luciferase reporter can be improved by normalizing to a control reporter, such as Renilla luciferase reporter, in the same sample. 产品介绍 The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly. This reagent lyses the cells and contains a substrate for firefly luciferase to produce firefly luciferase luminescence. Next, the Renilla Luciferase Reagent is added to the same well. It quenches the firefly luciferase luminescence and provides the substrate for renilla luciferase to produce renilla luciferase luminescence. The light production of both reactions can be conveniently measured on a luminometer. This assay system has several features: • Sensitive: highly sensitive detection of firefly luciferase activity and Renilla luciferase activity. • Stable: the luciferase signal output is stable for more than one hour, providing flexibility with regard to incubation time • High-throughput: simple homogeneous protocol minimizes handling steps to support high-throughput screening applications • Compatibility: works well with a variety of common media containing 0-10% serum and phenol red.
询价背景介绍 Firefly luciferase has been used as a sensitive reporter to study a wide range of biological responses. However the change of the expression of Firefly luciferase reporter can be due to a global effect instead of a specific effect. The accuracy of Firefly luciferase reporter can be improved by normalizing to a control reporter, such as Renilla luciferase reporter, in the same sample. 产品介绍 The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly. This reagent lyses the cells and contains a substrate for firefly luciferase to produce firefly luciferase luminescence. Next, the Renilla Luciferase Reagent is added to the same well. It quenches the firefly luciferase luminescence and provides the substrate for renilla luciferase to produce renilla luciferase luminescence. The light production of both reactions can be conveniently measured on a luminometer. This assay system has several features: • Sensitive: highly sensitive detection of firefly luciferase activity and Renilla luciferase activity. • Stable: the luciferase signal output is stable for more than one hour, providing flexibility with regard to incubation time • High-throughput: simple homogeneous protocol minimizes handling steps to support high-throughput screening applications • Compatibility: works well with a variety of common media containing 0-10% serum and phenol red.
询价背景介绍 Firefly luciferase has been used as a sensitive reporter to study a wide range of biological responses. However the change of the expression of Firefly luciferase reporter can be due to a global effect instead of a specific effect. The accuracy of Firefly luciferase reporter can be improved by normalizing to a control reporter, such as Renilla luciferase reporter, in the same sample. 产品介绍 The Dual Luciferase (Firefly-Renilla) Assay System is designed to be used for high-throughput, rapid quantitation of both Firefly and Renilla luciferases from a single sample in mammalian cell culture. The Firefly Luciferase Reagent is first added to the cells in medium directly. This reagent lyses the cells and contains a substrate for firefly luciferase to produce firefly luciferase luminescence. Next, the Renilla Luciferase Reagent is added to the same well. It quenches the firefly luciferase luminescence and provides the substrate for renilla luciferase to produce renilla luciferase luminescence. The light production of both reactions can be conveniently measured on a luminometer. This assay system has several features: • Sensitive: highly sensitive detection of firefly luciferase activity and Renilla luciferase activity. • Stable: the luciferase signal output is stable for more than one hour, providing flexibility with regard to incubation time • High-throughput: simple homogeneous protocol minimizes handling steps to support high-throughput screening applications • Compatibility: works well with a variety of common media containing 0-10% serum and phenol red.
询价背景介绍 The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. 产品介绍 Human chromodomain helicase DNA binding protein 2, or CHD2, (GenBank Accession No. NM_001271) a.a. 260-456(end), with N-terminal GST-tag, MW=49.3 kDa, expressed in an E. coli cell expression system.
询价背景介绍 The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. 产品介绍 Human chromodomain helicase DNA binding protein 2, or CHD2, (GenBank Accession No. NM_001271) a.a. 260-456(end), with N-terminal GST-tag, MW=49.3 kDa, expressed in an E. coli cell expression system.
询价产品介绍 HMT Assay Buffer 2 is optimized for assaying EZH1, EZH2, PRMT1, PRMT3, and PRMT5. This buffer is designed for BPS Bioscience's homogeneous (Alphascreen) assays. For BPS Bioscience's chemiluminescent assays, use cat. #52170.
询价
