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产品介绍 Human Receptor Activator of Nuclear FactorκB (RANK), also known as TRANCE Receptor and TNFRSF11A, (Genbank Accession No. NM_003839), a.a. 30-212, fused at the C-terminus to the Fc portion of human IgG1, with a C-terminal Avi-tag, expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using AviTag™ technology. MW = 49 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation
询价产品介绍 Human Receptor Activator of Nuclear FactorκB (RANK), also known as TRANCE Receptor and TNFRSF11A, (Genbank Accession No. NM_003839), a.a. 30-212, fused at the C-terminus to the Fc portion of human IgG1, with a C-terminal Avi-tag, expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using AviTag™ technology. MW = 49 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation
询价产品介绍 Human CEACAM, also known as Carcinoembryonic antigen-related cell adhesion molecule 1 or biliary glycoprotein, (GenBank Accession No. NM_ 001712), amino-acids 35-428, with C-terminal Avi-His-tag, MW = 47 kDa, expressed in HEK293 cells. This protein is enzymatically biotinylated using Avi-tag™ technology. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
询价产品介绍 Human CEACAM, also known as Carcinoembryonic antigen-related cell adhesion molecule 1 or biliary glycoprotein, (GenBank Accession No. NM_ 001712), amino-acids 35-428, with C-terminal Avi-His-tag, MW = 47 kDa, expressed in HEK293 cells. This protein is enzymatically biotinylated using Avi-tag™ technology. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
询价产品介绍 Human Keap1, also known as Kelch-like ECH-associated protein, INRF2, KIAA0132, and KLHL19, GenBank Accession No. NM_203500, a.a. 321-609 expressed in an E. coli cell expression system. MW = 32.6 kDa
询价产品介绍 Human Keap1, also known as Kelch-like ECH-associated protein, INRF2, KIAA0132, and KLHL19, GenBank Accession No. NM_203500, a.a. 321-609 expressed in an E. coli cell expression system. MW = 32.6 kDa
询价背景介绍 Caspase-7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. 产品介绍 Human Caspase-7 (full length) (GenBank Accession No. NM_001227) with C-terminal His-tag, MW= 35.1 kDa, expressed in an E. coli expression system. Active caspase 7 is a heterotetramer consisting of two copies each of ~20 kDa and ~11 kDa subunits.
询价背景介绍 Caspase-7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. 产品介绍 Human Caspase-7 (full length) (GenBank Accession No. NM_001227) with C-terminal His-tag, MW= 35.1 kDa, expressed in an E. coli expression system. Active caspase 7 is a heterotetramer consisting of two copies each of ~20 kDa and ~11 kDa subunits.
询价背景介绍
Luciferase is the general term given to a class of oxidative enzymes that catalyze reactions that give off light, a process known as bioluminescence. In biology, researchers can take advantage of this reaction and use it as a readout for various biological processes. This has perhaps been exploited most in luciferase reporter cell lines where a promoter region from a gene of interest is placed immediately upstream of the coding sequence for luciferase. In this system, transcriptional activation of the gene of interest leads to a level of luciferase expression that is proportional to the level of gene activation.
The level of activation is then assessed by lysing the cells and adding the luciferase substrate, D-luciferin. Using the One-Step Luciferase Assay System from BPS Bioscience, this is accomplished in a single step as the lysis buffer has been formulated with D-luciferin. After a brief incubation period, bioluminescence is read on a luminometer.
产品介绍
The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features:
• Sensitive - highly sensitive detection of firefly luciferase activity.
• Stable - the signal output is stable for more than two hours, providing flexibility with regard to incubation time
• Convenient - simple one-step, homogeneous protocol.
• High-throughput - one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications
• Compatibility - works well with a variety of common media containing 0-10% serum and phenol red.
• Instrumentation - does not require a luminometer with injectors.
