服务平台拥有丰富的技术服务经验，一流的仪器设备，先进的检测手段和严格的质量控制体系，力求为全球药物研发机构提供优质高效的技术服务
背景介绍 3-methylcytosine arises by erroneous methylation of DNA through spontaneous exposure to endogenous S-adenosyl methionine (SAM). Since the N3-position in cytosine is protected in double stranded DNA, this phenomenon mainly occurs in single stranded DNA. The resulting 3-mC is toxic and mutagenic and increased levels of 3-mC seem to impair cell proliferation. Therefore, it has to be repaired by the cell. This is established by oxidative demethylation, which is catalyzed by the AlkB protein. 产品介绍 Polyclonal antibody raised in rabbit against 3-methylcytosine (3-mC) conjugated to BSA.
询价背景介绍 Red fluorescent protein cloned from Discosoma coral (DsRed or drFP583) is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish). 产品介绍 Affinity purified Anti-Red Fluorescent Protein (RFP), clone RF5R, Mouse IgG1. Recognizes native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DeRed, mCherry, mOrange, etc. Purified from mouse ascites fluid. Applications: Western Blot (1:1000-3000), Dot Blot, ELISA, Immunocytochemistry (1:500-2000), Immunoprecipitation. Optimal dilutions/concentrations should be determined by the end user. Precited M.W. of RFP by Western blot is ~26 kDa.
询价背景介绍 Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. 产品介绍 Affinity purified Anti-Myc, clone Myc.A7/M19, Mouse IgG1. Recognizes both N-terminal or C-terminal Myc-tagged fusion proteins. Applications: Western Blot (1:1000 - 3000), Dot Blot, ELISA, Immunocytochemistry (1:500-2000), Immunoprecipitation (1:200), In Situ Hybridization. Optimal dilutions/concentrations should be determined by the end user.
询价背景介绍 Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. 产品介绍 Affinity purified monoclonal Anti-His-Tag, clone HIS.H8/ EH158, mouse IgG2b. Recognizes His-tagged recombinant proteins or His-tagged proteins overexpressed in cells. Applications: Western blotting (1:1000), ICC (1:200), IP (1:200), ELISA, Electrophoretic mobility shift assay. Optimal dilutions/concentrations should be determined by the end user.
询价背景介绍 Hemagglutinin is an antigenic glycoprotein found on the surface of the influenza viruses, as well as many other bacteria and viruses. It is responsible for binding the virus to the cell that is being infected. The name hemagglutinin comes from the protein's ability to cause red blood cells (erythrocytes) to agglutinate in vitro. The HA tag is derived from the HA molecule corresponding to a.a. 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. 产品介绍 Affinity purified anti-HA-tag clone HA.C5/C10, mouse IgG3. Recognizes the N-terminal, C-terminal or internal HA- tagged fusion proteins. Applications: Western Blot (1:1000), Dot Blot, ELISA, Immunocytochemistry (1:200), Immunoprecipitation (1:200), In Situ Hybridisation (1:500-2000). Optimal dilutions/concentrations should be determined by the end user.
询价背景介绍 GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins. 产品介绍 Affinity purified monoclonal Anti-GST-Tag, clone GST.B6, mouse IgG2a. Recognizes native and denatured forms of purified GST or GST fusion proteins. Applications: Western Blot (1:1000-3000), Dot Blot, ELISA, Immunocytochemistry (1:500-2000), Immunoprecipitation. Optimal dilutions/concentrations should be determined by the end user.
询价背景介绍 Green fluorescent protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. 产品介绍 Affinity purified Anti-GFP (Green Fluorescent Protein) Tag clone GF28R, mouse IgG1. Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP. Applications: Western Blot(1:1000-3000), Dot Blot, ELISA, Immunocytochemistry(1:500-2000), Immunoprecipitation. Optimal dilutions/concentrations should be determined by the end user.
询价背景介绍 Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Antibodies to GADPH are often used as "loading controls" because the enzyme is so ubiquitous. 产品介绍 Affinity purified Anti-GAPDH clone GA1R, mouse IgG1. Recognizes native and denatured forms of GAPDH (~37 kDa). Applications: Western Blotting(1:1000-10000), Dot Blot, ELISA, Immunocytochemistry, Immunofluorescence assays. Optimal dilutions/concentrations should be determined by the end user.
询价产品介绍 Affinity purified Anti-ERK1/2, clone E31R, mouse IgG1. Recognizes human ERK1 and ERK2 proteins. Application: Western blotting (1:1000). Optimal dilutions/concentrations should be determined by the end user. Predicted molecular weight: 41, 43 kDa.
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