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背景介绍 Calcium-activated chloride channels (CaCCs) are major regulators in numerous physiological processes including sensory transduction, epithelial secrection, cardiac and neutonal excitation, and smooth muscle contraction. TMEM16A (ANO1), a member of a family of putative plasma membrane proteins, is identified as a CaCC that is activated by intracellular Ca2+ and Ca2+-mobilizing stimuli. It has eight putative transmembrane segments without domains evidently involved in calcium regulation. The relative permeability of TMEM16A to monovalent anions is NO3–> I–>Br–> Cl– > F–. 产品介绍 Stable, recombinant HEK293 cell line expressing human TMEM16A (transmembrane protein 16A, also called anoctamin 1, calcium-activated chloride channel (ANO1), accession number NM_018043).
询价背景介绍 TRPC3 channel belongs to the transient receptor potential channel (TRP) superfamily which is divided into seven main subfamilies. TRP channels share a similar architecture of six-transmembrane domains and function as tetramers. Most channels are permeable to calcium and monovalent cations (Vassort et al., 2009). Specifically, TRPC3 channel belongs to a canonical TRP (TRPC) subfamily that is involved in calcium entry. TRPC3 is activated through phospholipase C-linked receptors. When expressed in HEK293 cells, TRPC3 behaves as a receptor activated channel with constitutive activity that cannot be further increased by store depletion (Trebak et al., 2002). 产品介绍 Stable, recombinant HEK293 cell line expressing human TRPC3 (transient receptor potential cation channel, subfamily C, member 3, accession number NM_003305).
询价产品介绍 Stable, recombinant HEK293 cell line expressing human TRPC7 (transient receptor potential cation channel, subfamily C, member 7, accession number NM_020389).
询价产品介绍 The NF-κB reporter (Luc)-Raw 264.7 cell line is designed for monitoring nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.
询价背景介绍 Human Angiotensin converting enzyme 2 (ACE2), also known as ACEH, is an integral membrane protein found in the outer space of cells in the lungs, arteries, heart, kidney, and intestines. ACE2 serves as the entry point into cells for some coronaviruses, including the SARS-CoV-2 virus that is responsible for the COVID-19 pandemic. 产品介绍 Recombinant clonal stable CHO cell line constitutively expressing full length human ACE2, Genbank #NM_021804.3). Surface expression of ACE2 was confirmed by flow cytometry.
询价背景介绍 Human Angiotensin converting enzyme 2 (ACE2), also known as ACEH, is an integral membrane protein found in the outer space of cells in the lungs, arteries, heart, kidney, and intestines. ACE2 serves as the entry point into cells for some coronaviruses, including the SARS-CoV-2 virus that is responsible for the COVID-19 pandemic. 产品介绍 Recombinant clonal stable HeLa cell line constitutively expressing full length human ACE2, Genbank #NM_021804.3). Surface expression of ACE2 was confirmed by flow cytometry.
询价背景介绍 Human Angiotensin converting enzyme 2 (ACE2), also known as ACEH, is an integral membrane protein found in the outer space of cells in the lungs, arteries, heart, kidney, and intestines. ACE2 serves as the entry point into cells for some coronaviruses, including the SARS-CoV-2 virus that is responsible for the COVID-19 pandemic. 产品介绍 Recombinant clonal stable HEK293 cell line constitutively expressing full length human ACE2, Genbank #NM_021804.3). Surface expression of ACE2 was confirmed by flow cytometry.
询价背景介绍 Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine stimulating the production of granulocytes and monocytes from bone marrow precursors. GM-CSF had pro-inflammatory functions and is a therapeutic target in autoimmune disease. GM-CSF signals through JAK2/STAT5 and stimulates the expression of STAT5 target genes. 产品介绍 The STAT5 Reporter (Luc)-U937 cell line is designed for monitoring STAT5 signal transduction pathway in the U937 cell line. It contains a firefly luciferase gene driven by the STAT5 response element located upstream of the minimal TATA promoter. After activation by GM-CSF, endogenous STAT5 binds to the DNA response elements, inducing transcription of the luciferase reporter gene.
询价背景介绍 The T-Cell Receptor (TCR) is found on the surface of T-cells and is responsible for recognizing antigens bound to MHC (Major Histocompatibility Complex) molecules. Activation of the TCR results in activation of downstream NFAT signaling. The TCR consists of a heterodimer of two different protein chains, of which the alpha (α) and beta (β) chains are the predominant chains. CRISPR/Cas9 genome editing was utilized to remove the TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) regions of the α and β chains, resulting in loss of TCR expression. 产品介绍 The TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains have both been genetically removed by CRISPR/Cas9 genome editing from recombinant Jurkat cells constitutively expressing the firefly luciferase gene under the control of NFAT response elements.
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