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背景介绍 Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing HCT116 cell lines can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines. 产品介绍 This cell line is a clonal derivative from the Cas9-Expressing HCT116 Cell Pool (BPS Bioscience, #78073). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag.
询价背景介绍 Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing A549 cell lines can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines. 产品介绍 This cell line is a clonal derivative from the Cas9-Expressing A549 Cell Pool (BPS Bioscience, #78072). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a low-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag.
询价背景介绍 Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing A549 cell lines can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines. 产品介绍 This cell line is a clonal derivative from the Cas9-Expressing A549 Cell Pool (BPS Bioscience, #78072). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag.
询价背景介绍 In contrast to other Nectins, which are found extensively in adult tissues, Nectin4 is abundant during fetal development but declines in adult life. Its expression, however, returns specifically in lung, breast, pancreas and ovarian cancers. It was shown that Nectin4 can modulate the expression of epithelial-mesenchymal transition-related proteins via the PI3K/AKT pathway. In addition, the recent study demonstrated that Nectin4 is a cancer-specific TIGIT ligand and its expression is also associated with poor prognostic features, suggesting it could be an efficient target for cancer immunotherapy. 产品介绍 Recombinant human Nectin4-CHO K1 cell line stably expressing a full length human Nectin4/PVRL4 receptor (GenBank Accession: NM_030916.2). Surface expression of hNectin4 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of hNectin4 expression (High, Medium, Low) to mimic different stages of cancer target cells with various Nectin4 expression levels.
询价背景介绍 In contrast to other Nectins, which are found extensively in adult tissues, Nectin4 is abundant during fetal development but declines in adult life. Its expression, however, returns specifically in lung, breast, pancreas and ovarian cancers. It was shown that Nectin4 can modulate the expression of epithelial-mesenchymal transition-related proteins via the PI3K/AKT pathway. In addition, the recent study demonstrated that Nectin4 is a cancer-specific TIGIT ligand and its expression is also associated with poor prognostic features, suggesting it could be an efficient target for cancer immunotherapy. 产品介绍 Recombinant human Nectin4-CHO K1 cell line stably expressing a full length human Nectin4/PVRL4 receptor (GenBank Accession: NM_030916.2). Surface expression of hNectin4 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of hNectin4 expression (High, Medium, Low) to mimic different stages of cancer target cells with various Nectin4 expression levels.
询价背景介绍 In contrast to other Nectins, which are found extensively in adult tissues, Nectin4 is abundant during fetal development but declines in adult life. Its expression, however, returns specifically in lung, breast, pancreas and ovarian cancers. It was shown that Nectin4 can modulate the expression of epithelial-mesenchymal transition-related proteins via the PI3K/AKT pathway. In addition, the recent study demonstrated that Nectin4 is a cancer-specific TIGIT ligand and its expression is also associated with poor prognostic features, suggesting it could be an efficient target for cancer immunotherapy. 产品介绍 Recombinant human Nectin4-CHO K1 cell line stably expressing a full length human Nectin4/PVRL4 receptor (GenBank Accession: NM_030916.2). Surface expression of hNectin4 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of hNectin4 expression (High, Medium, Low) to mimic different stages of cancer target cells with various Nectin4 expression levels.
询价背景介绍 L-Trp is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. TDO is upregulated in many tumors, providing cancer cells with an avenue for immune evasion. 产品介绍 The TDO Cellular Activity QuickDetectTM Supplements are designed to complement the hTDO-HEK293 Recombinant Cell Line (BPS Cat. #60534) or other TDO-expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of TDO and for the analysis and detection of tryptophan 2,3 dioxygenase (TDO)- catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.
询价背景介绍 L-Trp is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. TDO is upregulated in many tumors, providing cancer cells with an avenue for immune evasion. 产品介绍 The TDO Cellular Activity QuickDetectTM Supplements are designed to complement the hTDO-HEK293 Recombinant Cell Line (BPS Cat. #60534) or other TDO-expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of TDO and for the analysis and detection of tryptophan 2,3 dioxygenase (TDO)- catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.
询价背景介绍 L-Trp is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L -Trp that have immunosuppressive functions. IDO2 is upregulated in many tumors, providing cancer cells with an avenue for immune evasion. 产品介绍 The IDO2 Cellular Activity QuickDetect™ Supplements are designed to complement the hIDO2-HEK293 Recombinant Cell Line (BPS Cat. #60532) or other IDO2-expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of IDO2 and for the analysis and detection of indoleamine 2,3 dioxygenase (IDO2)-catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.
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