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产品介绍 The EZH2 (Y641F) TR-FRET Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641F)/EED/SUZ12/RbAp48/ AEBP) in a homogeneous 384 reaction format. This FRET-based assay requires no timeconsuming washing steps, making it especially suitable for high throughput screening applications. The EZH2 (Y641F) TR-FRET Assay Kit comes in a convenient format, with histone H3 peptide substrate, a Tb-labeled antibody against methylated K27 residue of Histone H3, S-adenosylmethionine, methyltransferase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and purified EZH2 (Y641F) complex for 384 enzyme reactions. The key to the EZH2 (Y641F) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for 240 minutes. Next, antibody is added. Finally, dye-labeled acceptor is added followed by fluorescence reading.
询价产品介绍 The 4x HDM Incomplete Assay Buffer does not contain _x0001_-ketoglutarate and provides a more accurate background measure than using a no-enzyme control. It has been optimized for use with the following BPS Bioscience histone demethylase assay kits: • FBXL10 (KDM2B) Homogeneous Assay Kit, BPS Cat. #50610 • FBXL11 (KDM2A) Homogeneous Assay Kit, BPS Cat. #50611 • JARID1A (KDM5A) Homogeneous Assay Kit, BPS Cat. #50510 • JARID1B (KDM5B) Homogeneous Assay Kit, BPS Cat. #50512 • JARID1C (KDM5C) Homogeneous Assay Kit, BPS Cat. #50511 • JHDM1D (KDM7A) Homogeneous Assay Kit, BPS Cat. #50420 • JMJD1A (KDM3A) Homogeneous Assay Kit, BPS Cat. #50412 • JMJD2A (KDM3A) Homogeneous Assay Kit, BPS Cat. #50413 • JMJD2B (KDM4B) Homogeneous Assay Kit, BPS Cat. #50414 • JMJD2C (KDM4C) Homogeneous Assay Kit, BPS Cat. #50415 • JMJD2E (KDM4DL) Homogeneous Assay Kit, BPS Cat. #50417 • JMJD3 (KDM6B) Homogeneous Assay Kit, BPS Cat. #50416 • PHF8 (KDM7B) Homogeneous Assay Kit, BPS Cat. #50515 • UTX (KDM6A) Homogeneous Assay Kit, BPS Cat. #50516
询价产品介绍 The 4x HDM Assay Buffer 4 is optimized for use with the following BPS Bioscience histone demethylase assay kits: • JMJD2A (KDM4A) Homogeneous Assay Kit, BPS Cat. #50413 • JHDM1D (KDM7A) Homogeneous Assay Kit, BPS Cat. #50420
询价产品介绍 The 4x HDM Assay Buffer 3 is optimized for use with the following BPS Bioscience histone demethylase assay kits: • JMJD2C (KDM4C) Homogeneous Assay Kit, BPS Cat. #50415 • JMJD2E (KDM4DL) Homogeneous Assay Kit, BPS Cat. #50417 • JMJD3 (KDM6B) Homogeneous Assay Kit, BPS Cat. #50416 • PHF8 (KDM7B) Homogeneous Assay Kit, BPS Cat. #50515
询价产品介绍 The 4x HDM Assay Buffer 2 is optimized for use with the following BPS Bioscience histone demethylase assay kits: • JARID1A (KDM5A) Homogeneous Assay Kit, BPS Cat. #50510 • JARID1B (KDM5B) Homogeneous Assay Kit, BPS Cat. #50512 • JMJD1A (KDM3A) Homogeneous Assay Kit, BPS Cat. #50412 • JMJD2B (KDM4B) Homogeneous Assay Kit, BPS Cat. #50414
询价产品介绍 The 4x HDM Assay Buffer 1 is optimized for use with the following BPS Bioscience histone demethylase assay kits: • JARID1C (KDM5C) Homogeneous Assay Kit, BPS Cat. #50511
询价产品介绍 Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A (a.a. 2-130(end)), H2B (a.a. 2-126(end)), H3 (a.a. 2-137(end)), and H4 (a.a. 2-103(end)), expressed in an E. coli expression system. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Each histone protein has an N-terminal His-tag. Total histone MW = 113.8 kDa.
询价产品介绍
The 4x Demethyltransferase Detection Buffer 1 is optimized for use with the following BPS Bioscience JMJD2 assay kits:
• JMJD2A Homogeneous Assay Kit, #50413
• JMJD2B Homogeneous Assay Kit, #50414
• JMJD2C Homogeneous Assay Kit, #50415
• JMJD2E Homogeneous Assay Kit, #50417
• JMJD2C Chemiluminescent Assay Kit, #50405
• LSD1 Homogeneous Assay Kit, #50108.
产品介绍 The DOT1L Chemiluminescent Assay Kit is designed to measure DOT1L activity for screening and profiling applications. The key to the DOT1L Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K79 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for two hours. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
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