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产品介绍 Human PKAcα, GenBank Accession No. NM_002730, 2-351 (end), with N-terminal His-Tag, MW = 42 kDa, expressed in an E.coli expression system.
询价背景介绍 Mammalian PTPases can be subdivided into 1 of 2 broad categories: transmembrane receptor PTPases and intracellular PTPases. PTPN11 is one of the 2 closely related mammalian intracellular PTPases whose sequences encode 2 tandem SRC homology 2 (SH2) domains that are located at the amino-terminal side of a single PTPase catalytic domain. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. 产品介绍 Human recombinant SHP-2, also known as PTPN11 (protein tyrosine phosphatase N11), full length (a.a. 2-593 (end) with N-terminal His-tag, Genbank accession number NM_002834, MW=70 kDa, expressed in an E. coli expression system.
询价产品介绍 Human FCGR3B, known as CD16b, and FCG3, GenBank Accession No. NM_000570.4, a.a. 20-208, with C-terminal Avi™-His-Tag, expressed in a HEK293 cell expression system. This protein runs at a higher MW by SDS-PAGE due to glycosylation. MW = 25 kDa.
询价产品介绍 Human FCGR3A, known as Fc gamma RIIIA, and CD16a, GenBank Accession No. NM_001127593.1, a.a. 17-208, with N-terminal Avi™-His-Tag, expressed in a HEK293 cell expression system. This protein runs at a higher MW by SDS-PAGE due to glycosylation. MW = 25 kDa.
询价产品介绍 Human secreted programmed cell death 1 (PD-1) also known as PDCD1, SLEB2, CD279 and HPD-L, GenBank Accession No. NM_005018, a.a 25-167, fused at the C-terminus to the Fc portion of human IgG1, Eu-labeled, expressed in a HEK293 cell expression system. MW = 43 kDa.
询价产品介绍 Human HMGB1 also known as high-mobility group protein 1, and amphoterin, GenBank Accession No. NM_002128, a.a. 2-215, with C-terminal Avi-His-Tag, and expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using AviTagTM technology. MW = 25 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
询价产品介绍 Mouse secreted B7-1, also known as CD80. GenBank Accession No. NM_009855, a.a. 38-246 with C-terminal FLAG-Avi-His-Tag, expressed in a HEK293 cell expression system. MW = 28 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
询价背景介绍 The protein kinase c (PKC)/ Ca2+ response pathway leads to activation of the transcription factor nuclear factor of activator T cells (NFAT). NFAT is regulated by Ca2+ and the Ca2+/calmodulin-dependent serine phosphatase calcineurin. NFAT proteins are phosphorylated and reside in the cytoplasm in resting cells; upon stimulation, they are dephosphorylated by calcineurin, translocate to the nucleus, and induce gene expression. 产品介绍 Mouse secreted CTLA4, with C-terminal Avi-Tag fused at the C-terminus with the Fc portion of human IgG1 and biotin labeled, also known as Cytotoxic T-lymphocyte-associated protein 4 and CD152. GenBank Accession No. NM_009843, a.a. 36-161 expressed in a HEK293 cell expression system. The protein is enzymatically biotinylated using AviTag™ technology. MW = 42 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
询价背景介绍 PARP1 and PARP2 enzymes are known to bind damaged DNA through their DNA-binding domains. Binding to DNA activates these enzymes and in the presence of NAD+, PARP1 and PARP2 ribosylate themself (autoribosylation), leading to their dissociation from the DNA due to the accumulated negative charge of the ribosyl polymer. In the presence of some inhibitors, however, these PARPs remain bound to the DNA, a phenomenon termed trapping. Trapped PARP-DNA complexes have been shown to be highly cytotoxic to cancer cells, therefore such inhibitors may be desirable for cancer treatment. 产品介绍 The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a kit designed to measure DNA complex formations of PARP1 and PARP2 enzymes in a high throughput screening assay using fluorescence polarization (FP). The key to the PARPtrap™ Combo Assay Kit are the fluorescent-labeled DNA probes recognized by PARP1 and PARP2. In the absence of ribosylation, PARP1/2 binds to the DNA fluorescent probe, forming a large complex and resulting in the emission of highly polarized light. However, after autoribosylation, PARP1/2 dissociates from the oligonucleotide duplex, which then rotates freely, emitting less polarized light (Fig. 1). Addition of a PARP inhibitor results in trapping of PARP1/2 to the fluorescent-labeled DNA, and increases the FP signal in a dose dependent manner. The combo kit is particularly useful to directly and specifically compare, in a single assay, the effect and potency of a compound on PARP1 and PARP2. The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a homogeneous fluorescence polarization assay. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.
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