VEGFR3(FLT4) Kinase Assay Kit

背景介绍 Vascular endothelial growth factor receptor 3 (VEGFR3), also called Fms-Related Tyrosine Kinase 4 (FLT4), is a tyrosine kinase (TK) receptor for VEGFs that plays a central role in tumor angiogenesis; therefore, the inhibition of VEGFR3 is a promising therapeutic strategy for inhibiting angiogenesis and tumor growth. 产品介绍 The VEGFR3 Kinase Assay Kit is designed to measure VEGFR3 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.

RIPK2 Kinase Assay Kit

背景介绍 RIPK2 (receptor-interacting protein kinase 2) has been shown to mediate the activation of pro-inflammatory signals by NODs (Nucleotide-binding oligomerization domain-containing proteins),suggesting RIPK2 may be a promising therapeutic target in autoimmune and inflammatory diseases. 产品介绍 The RIPK2 Kinase Assay Kit is designed to measure RIPK2 kinase activity for screening and profiling applications using ADP-Glo® Kinase Assay as a detection reagent.

PDE3A Assay Kit

背景介绍 Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE3A, also known as cGMP-inhibited phosphodiesterase, has been implicated in cardiovascular function and fertility. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light. 产品介绍 The PDE3A Assay Kit is designed for identification of PDE3A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE3A to the binding agent. REPLACES #60330

Dog PDE3A Assay Kit

背景介绍 Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE3A, also known as cGMP-inhibited phosphodiesterase, has been implicated in cardiovascular function and fertility. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light. 产品介绍 The Dog PDE3A Assay Kit is designed for identification of inhibitors of canine PDE3A using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Dog PDE3A to the binding agent.

CD112R:CD112[Biotinylated] Inhibitor Screening Assay Kit

背景介绍 CD112 (Poliovirus receptor-related 2, PVRL2), widely expressed on antigen-presenting cells and tumor cells, is the high affinity ligand of CD112R (Poliovirus receptor related immunoglobulin domain containing, PVRIG). CD112-CD112R interaction is a positive immune checkpoint that enhances human T cell response and it has emerged as an attractive therapeutic target for oncology. TIGIT (T cell immunoreceptor with Ig and ITIM domains) and CD226 (also called DNAM-1, DNAX Accessory Molecule-1) are other ligands of CD112. 产品介绍 The CD112R:CD112[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD112R-CD112. The key to this kit is the high sensitivity of detection of biotin-labeled CD112 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD112R is coated on a 96-well plate. Next, CD112 is incubated with CD112R on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

STAT3 Reporter Kit (STAT3 Signaling Pathway)

背景介绍 STAT3 (Signal Transducer and Activator of Transcription 3) is a transcription factor which is activated through phosphorylation at Tyrosine 705 in response to various cytokines and growth factors. The phosphorylated STAT3 forms homodimers or heterodimers with STAT1 and the dimers translocated to the nucleus where DNA binding/promoter induction occurs. STAT3 plays an important role in cellular processes such as cell cycle progression and apoptosis. 产品介绍 The STAT3 Reporter kit is designed for monitoring the activity of the STAT3 signaling pathway in cultured cells. The kit contains transfection-ready STAT3 luciferase reporter vector. This reporter contains a firefly luciferase gene under the control of STAT3-responsive element located upstream of a minimal promoter. The STAT3 reporter is premixed with constitutively expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency.
The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.

Blocking Buffer 2 (BPS Immunotherapy Assay Kits)

BCMA:APRIL[Biotinylated] Inhibitor Screening Assay Kit

背景介绍 A proliferation-inducing ligand (APRIL, TNSF13 or CD256) and B-cell maturation antigen (BCMA, also known as TNFRSF17 or CD269) create a signaling pathway that participates in the regulation of B-cell development, autoimmunity and long-term plasma cell survival. APRIL expression is significantly elevated in multiple myeloma (MM) and it has been demonstrated that activation of BCMA by APRIL promotes tumor growth, chemoresistance and immunosuppression in the bone marrow microenvironment. Anti-APRIL monoclonal antibodies have been recently progressed to clinical trials for MM. 产品介绍 The BCMA:APRIL[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of BCMA:APRIL. The key to this kit is the high sensitivity of detection of biotin-labeled APRIL by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, BCMA is coated on a 96-well plate. Next, biotinylated APRIL is incubated with BCMA on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

PRMT7 Homogeneous Assay Kit (Automethylation)

产品介绍 The PRMT7 Homogeneous Assay Kit (Automethylation) is designed to measure PRMT7 automethylation for screening and profiling applications. Automethylation of PRMT7 plays a key role in inducing the epithelial-mesenchymal transition program and in promoting the migratory and invasive behavior of breast cancer cells. The key to the PRMT7 Homogeneous Assay Kit (Automethylation) is a highly specific antibody that recognizes methylated PRMT7. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT7 enzyme is incubated with SAM. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Note: To study methylation of histone proteins by PRMT7, we recommend using our PRMT7 Homogeneous Assay Kit (H2b), #79720.