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产品介绍 Human Ten-eleven translocation 1 protein (TET1), methylcytosine dioxygenase TET1, Leukemiaassociated protein with a CXXC domain, CXXC-type zinc finger protein 6, GenBank Accession # NM_030625, a.a. 1418-2136(end) with N-terminal FLAG-tag, MW = 80 kDa, expressed in Sf9 cells via a baculovirus expression system.
询价产品介绍 Human Ten-eleven translocation 1 protein (TET1), methylcytosine dioxygenase TET1, Leukemiaassociated protein with a CXXC domain, CXXC-type zinc finger protein 6, GenBank Accession # NM_030625, a.a. 1418-2136(end) with N-terminal FLAG-tag, MW = 80 kDa, expressed in Sf9 cells via a baculovirus expression system.
询价背景介绍 GCN5 (also known as KAT2A) is involved in various cellular events, and its dysfunction is linked to a number of human diseases including cancers and diabetes. GCN5 is a histone acetyltransferase that functions primarily as a transcriptional activator. It also functions as a repressor of NF-κappa-B by promoting ubiquitination of the NF-κappaB subunit RelA in a HAT-independent manner. 产品介绍 The GCN5 Chemiluminescent Assay Kit is an enzyme-linked immunosorbent assay (ELISA) designed to screen for inhibitors of GCN5. The GCN5 Chemiluminescent Assay Kit is based on the GCN5 enzyme transferring an acetyl group from an acetyl donor (acetyl CoA) to a histone substrate. The acetylated histone is recognized by a highly specific primary antibody, followed by an HRP-labeled secondary antibody. The chemiluminescence produced by HRP can be measured using a chemiluminescence reader.
询价背景介绍 HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. Fluorescence is therefore directly related to HDAC activity. 产品介绍 The Fluorogenic HDAC6 Assay Kit is a complete assay system designed to measure histone deacetylase 6 (HDAC6) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC6 activity measurements. In addition, the kit includes purified HDAC6 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC6 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.
询价背景介绍 HDACs regulate cellular processes by catalyzing the hydrolysis of an acetyl group from acetyllysines in modified proteins. In the HDAC assay, fluorescent-dye molecules are attached to a peptide containing acetyllysine. Attachment to the peptide quenches the fluorescence of the dye. After treatment of the peptide with an HDAC, the reaction is mixed with a development solution that is specific for nonacetylated lysines. If the acetyl group has been removed from the lysine by the HDAC, this solution will release the dye allowing for fluorescence. Fluorescence is therefore directly related to HDAC activity. 产品介绍 The Fluorogenic HDAC6 Assay Kit is a complete assay system designed to measure histone deacetylase 6 (HDAC6) activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC6 activity measurements. In addition, the kit includes purified HDAC6 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC6 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with purified HDAC enzyme. The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.
询价产品介绍 Human HDAC7 , GenBank Accession No. AY302468, a.a. 518-end, with N-terminal GST-tag, MW= 78 kDa, expressed in baculovirus expression system.
询价产品介绍 Human HDAC7 , GenBank Accession No. AY302468, a.a. 518-end, with N-terminal GST-tag, MW= 78 kDa, expressed in baculovirus expression system.
询价产品介绍 Human HDAC7 , GenBank Accession No. AY302468, a.a. 518-end, with N-terminal GST-tag, MW= 78 kDa, expressed in baculovirus expression system.
询价产品介绍 Human CRTAM, also known as Cytotoxic and Regulatory T-Cell Molecule, GenBank Accession No. NM_019604, amino acids 18-286, expressed in a HEK293 expression system. This protein has a C-terminal Avi-Tag fused to the Fc region of Human IgG1 and is enzymatically biotinylated using Avi-tag™ technology. MW = 59 kDa.
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