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ADP-Glo™ Assay+ASK1 Kinase Enzyme System Easily Screen and Profile ASK1 Kinase Inhibitors Includes kinase, substrate and reaction buffer Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
询价AMPK (A2/B1/G1) Kinase Enzyme System Easily Screen and Profile AMPK (A2/B1/G1) Kinase Inhibitors Includes kinase, substrate and reaction buffer Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
询价AMPK (A1/B1/G2) Kinase Enzyme System Easily Screen and Profile AMPK (A1/B1/G2) Kinase Inhibitors Includes kinase, substrate and reaction buffer Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
询价ALK6 Kinase Enzyme System Easily Screen and Profile ALK6 Kinase Inhibitors Includes kinase, substrate and reaction buffer Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
询价ALK2 Kinase Enzyme System Easily Screen and Profile ALK2 Kinase Inhibitors Includes kinase, substrate and reaction buffer Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
询价AKT3 Kinase Enzyme System Easily Screen and Profile AKT3 Kinase Inhibitors Includes kinase, substrate and reaction buffer Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
询价AKT2 Kinase Enzyme System Easily Screen and Profile AKT2 Kinase Inhibitors Includes kinase, substrate and reaction buffer Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
询价ACK Kinase Enzyme System Easily Screen and Profile ACK Kinase Inhibitors Includes kinase, substrate and reaction buffer Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
询价ADP-Glo™ Kinase Assay (ADP-Glo™ 激酶检测试剂盒) 是一个发光法激酶检测试剂盒,它检测激酶反应中所形成的ADP;ADP被转化成ATP,然后ATP 再被Ultra-Glo™ 萤光素酶转化成光。发光信号与激酶活性正相关。该试剂盒适合检测化合物对多种纯化激酶活性的影响,使之成为初级筛选和研究激酶作用的选择性的理想工具。ADP-Glo™ 激酶检测试剂盒可用于检测几乎任何可生成ADP的酶( 比如,激酶或ATP 酶) 的活性,ATP 浓度可高达1mM。 该试剂盒的操作有两个步骤: 1) 在激酶反应后,向其中加入一份与激酶反应体系等体积的ADP-Glo™ 试剂,使激酶反应终止,并消耗完剩余的ATP。 2) 加入激酶检测试剂,它在使ADP 转化成ATP的同时,还使用偶联的萤光素酶/ 萤光素反应来检测新合成的ATP。 ADP-Glo™ 激酶检测试剂盒具有很宽的动态范围,即使当ATP到ADP 的转化率很低的时候,也能产生强信号;非常适合于低活性激酶,比如受体酪氨酸激酶。该试剂盒产生的假阳性率最低,Z' 值大于0.8。
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