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Kinase Fusion Vector for NanoBRET™ Target Engagement Transfection-ready DNA to express full-length DYRK2 kinase fused to NanoLuc® Luciferase Measures kinase target engagement in live cells Use with NanoBRET™ TE Intracellular Kinase Assay, Adherent Format protocol
询价DYRK2 Kinase Enzyme System Easily Screen and Profile DYRK2 Kinase Inhibitors Includes kinase, substrate and reaction buffer Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
询价DYRK2 Kinase Enzyme System Easily Screen and Profile DYRK2 Kinase Inhibitors Includes kinase, substrate and reaction buffer Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
询价DYRK1B Kinase Enzyme System Easily Screen and Profile DYRK1B Kinase Inhibitors Includes kinase, substrate and reaction buffer Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
询价DYRK1B Kinase Enzyme System Easily Screen and Profile DYRK1B Kinase Inhibitors Includes kinase, substrate and reaction buffer Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
询价DYRK1A Kinase Enzyme System Easily Screen and Profile DYRK1A Kinase Inhibitors Includes kinase, substrate and reaction buffer Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
询价DYRK1A Kinase Enzyme System Easily Screen and Profile DYRK1A Kinase Inhibitors Includes kinase, substrate and reaction buffer Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity
询价高质量的脱氧核糖核苷酸(dNTPs) 对 cDNA 合成、测序和标记等重要反应的成功非常重要。Promega 公司的 dNTPs 中三磷酸核 苷酸超过 99%,浓度是 100mM,pH 为 7.5。
询价Dual-Luciferase®Reporter (DLR™) Assay System (DualLuciferase®双萤光素酶报告基因检测系统 ) 为双报告基因检测提供有效的手段。 在 DLR™ 检测中,萤火虫 (Photinus pyralis) 萤光素酶和海肾 (Renilla reniformis) 萤光素酶的活性可在单个样品中依次检测。首先检测萤火虫萤光素酶,将萤光素酶检测试剂II(LuciferaseAssay Reagent II, LAR II)加入样品中,产生的光信号至少持续1分钟。定量萤火虫萤光强度后,再在同一个样品中加入 Stop & Glo® 试剂,将上述反应终止,同时启动海肾萤光素酶反应。使用带有试剂自动注射器的发光检测仪,可在 6 秒内完成两个检测。 在 DLR™ 检测系统中,两个报告基因产生的线性检测的灵敏度均可达埃摩尔级 (<10-18),在实验宿主细胞内均无内源活性。而且,DLR™ 检测的一体化模式既可快速定量检测转染细胞,也可用于快速定量检测无细胞转录 / 翻译反应体系中的两个报告基因。Promega 推荐使用针对 DLR™ 检测系统进行过确证的发光检测仪,这些发光检测仪均带有 DLReady™ 标识。pGL6 萤光素酶报告基因载体是针对 DLR™ 检测系统而设计。组成型表达的海肾萤光素酶载体可与任何实验用萤火虫萤光素酶载体联合共转染。
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