Human BMP-4 (bone morphogenetic protein 4) protein is a key regulator of embryogenesis and supports the differentiation of embryonic stem cells and induced pluripotent stem cells. In addition, BMP4 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, and regulating neuronal and glial cell development. Highly pure 26 kDa disulfide–linked bioactive dimer comprised of the mature domain of human BMP-4 protein (animal-free and carrier protein-free).