Customized TR-FRET assay kit that combines the reagents necessary for the quantitative detection of five biomarkers in cell culture supernatants. Eak kit contains 100 points for each selected biomarker. Specific assay formulations for the selected target biomarkers can be found in the datasheets associated with the individual TR-FRET Biomarker Assay Kits. The kit does not include recombinant human standards, which must be purchased separately.